Part:BBa_K2717008:Experience

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Applications of BBa_K2717008

We used overlap-PCR and infusion tech to link our composite to pUC19. The whole pathway can be described in Figure 1.

Fig.19 Plasmids constructed for testing TGATG efficiency. This part is the experimental group

Then the plasmid is transfered into E.coli Trans5a. We use Flow Cytometer (FCM) to measure the bacteria's number and a relative fluorescence. Figure 2 is one of the FCM result graphs. The specific experiments and data processing methods can be found in our wiki. Finally, by comparing the final relative fluorescent intensity with that of Control group 2 (Part:BBa_K2717016), we found the TGATG causes a 13.2% reduction to pink fluorescent intensity. That is to say, using TGATG indeed reduces the expression of downstream gene.

Fig.20 FCM data and processing.

User Reviews

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